VUILLEMIN, Marlène; GRIMAUD, Florent; CLAVERIE, Marion; ROLLAND-SABATÉ, Agnes; GARNIER, Catherine; LUCAS, Patrick; MONSAN, Pierre; DOLS-LAFARGUE, Marguerite; REMAUD-SIMEON, Magali; MOULIS, Claire

doi:10.1016/j.carbpol.2017.09.056

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Carbohydrate Polymers. 2018, vol. 179, p. 10-18

Résumé en anglais

A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691 s(-1) and a half-life time of 111 h at 30 degrees C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1 x 10(9) g.mol(-1) that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.< Réduire

Mots clés

Dextransucrase

Gène codant

Dextran

Polysaccharide

Sulfate de dextrane

Propriété rhéologique

Oenococcus

Saccharase

Mots clés en anglais

Dextran

Bacterial Polysaccharide

Dextransucrase

Glucansucrase

Oenococcus Kitaharae