A dextran with unique rheological properties produced by the dextransucrase from Oenococcus kitaharae DSM 17330
Language
EN
Article de revue
This item was published in
Carbohydrate Polymers. 2018, vol. 179, p. 10-18
English Abstract
A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691 s(-1) and a half-life time of 111 h at 30 degrees C, the resulting recombinant ...Read more >
A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691 s(-1) and a half-life time of 111 h at 30 degrees C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1 x 10(9) g.mol(-1) that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.Read less <
Keywords
Dextransucrase
Gène codant
Dextran
Polysaccharide
Sulfate de dextrane
Propriété rhéologique
Oenococcus
Saccharase
English Keywords
Dextran
Bacterial Polysaccharide
Dextransucrase
Glucansucrase
Oenococcus Kitaharae
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