Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part II: Optimisation of the derivatization step
GEFFARD, M.
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
DOBRIJEVIC, M.
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Observatoire aquitain des sciences de l'univers [OASU]
< Leer menos
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Observatoire aquitain des sciences de l'univers [OASU]
Idioma
en
Article de revue
Este ítem está publicado en
Chemometrics and Intelligent Laboratory Systems. 2006, vol. 80, p. 186-197
Elsevier
Resumen en inglés
In a previous paper (part I), new strategy was used for raising antibodies against hapten (<300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising ...Leer más >
In a previous paper (part I), new strategy was used for raising antibodies against hapten (<300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising antibodies against histamine, they were characterized and used for ELISA measurements. Optimal detection conditions were determined for the histamine quantification by ELISA method. The present study investigates the derivatization step of the histamine by chloroethylnitrosourea (CENU). Five factors (2 qualitative: nature of the solvent and nature of the antibodies and 3 quantitative: pH, % of solvent and time of derivatization) have been considered. Optimal reaction conditions were established by calculation of a validated model.< Leer menos
Palabras clave en italiano
Experimental design
Box Behnken
Antibodies
Derivatization
Histamine quantification
Orígen
Importado de HalCentros de investigación