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Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part II: Optimisation of the derivatization step

hal.structure.identifierInstitut des Sciences Moléculaires de Marseille [ISM2]
dc.contributor.authorCLAEYS-BRUNO, M.
hal.structure.identifierOrganisation moléculaire (évolution et matériaux fluores) [OMEMF]
dc.contributor.authorVANDENABEELE-TRAMBOUZE, O.
hal.structure.identifierInstitut des Sciences Moléculaires de Marseille [ISM2]
dc.contributor.authorSERGENT, M.
hal.structure.identifierLaboratoire de physique des interactions ondes matières [LPIOM]
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorGEFFARD, M.
hal.structure.identifierLaboratoire de physique des interactions ondes matières [LPIOM]
dc.contributor.authorBODET, D.
hal.structure.identifierLaboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
hal.structure.identifierLaboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
hal.structure.identifierObservatoire aquitain des sciences de l'univers [OASU]
dc.contributor.authorDOBRIJEVIC, M.
hal.structure.identifierOrganisation moléculaire (évolution et matériaux fluores) [OMEMF]
dc.contributor.authorCOMMEYRAS, A.
hal.structure.identifierInstitut des Sciences Moléculaires de Marseille [ISM2]
dc.contributor.authorPHAN TAN LUU, R.
dc.date.issued2006
dc.identifier.issn0169-7439
dc.description.abstractEnIn a previous paper (part I), new strategy was used for raising antibodies against hapten (<300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising antibodies against histamine, they were characterized and used for ELISA measurements. Optimal detection conditions were determined for the histamine quantification by ELISA method. The present study investigates the derivatization step of the histamine by chloroethylnitrosourea (CENU). Five factors (2 qualitative: nature of the solvent and nature of the antibodies and 3 quantitative: pH, % of solvent and time of derivatization) have been considered. Optimal reaction conditions were established by calculation of a validated model.
dc.language.isoen
dc.publisherElsevier
dc.title.enMethodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part II: Optimisation of the derivatization step
dc.typeArticle de revue
dc.identifier.doi10.1016/j.chemolab.2005.06.007
dc.subject.halPhysique [physics]/Astrophysique [astro-ph]/Cosmologie et astrophysique extra-galactique [astro-ph.CO]
dc.subject.halPlanète et Univers [physics]/Astrophysique [astro-ph]
bordeaux.journalChemometrics and Intelligent Laboratory Systems
bordeaux.page186-197
bordeaux.volume80
bordeaux.peerReviewedoui
hal.identifierhal-00023304
hal.version1
hal.popularnon
hal.audienceNon spécifiée
dc.subject.itExperimental design
dc.subject.itBox Behnken
dc.subject.itAntibodies
dc.subject.itDerivatization
dc.subject.itHistamine quantification
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00023304v1
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