Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part II: Optimisation of the derivatization step
GEFFARD, M.
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
DOBRIJEVIC, M.
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Observatoire aquitain des sciences de l'univers [OASU]
< Réduire
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Observatoire aquitain des sciences de l'univers [OASU]
Langue
en
Article de revue
Ce document a été publié dans
Chemometrics and Intelligent Laboratory Systems. 2006, vol. 80, p. 186-197
Elsevier
Résumé en anglais
In a previous paper (part I), new strategy was used for raising antibodies against hapten (<300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising ...Lire la suite >
In a previous paper (part I), new strategy was used for raising antibodies against hapten (<300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising antibodies against histamine, they were characterized and used for ELISA measurements. Optimal detection conditions were determined for the histamine quantification by ELISA method. The present study investigates the derivatization step of the histamine by chloroethylnitrosourea (CENU). Five factors (2 qualitative: nature of the solvent and nature of the antibodies and 3 quantitative: pH, % of solvent and time of derivatization) have been considered. Optimal reaction conditions were established by calculation of a validated model.< Réduire
Mots clés en italien
Experimental design
Box Behnken
Antibodies
Derivatization
Histamine quantification
Origine
Importé de halUnités de recherche