Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part II: Optimisation of the derivatization step
GEFFARD, M.
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
DOBRIJEVIC, M.
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Observatoire aquitain des sciences de l'univers [OASU]
< Reduce
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Observatoire aquitain des sciences de l'univers [OASU]
Language
en
Article de revue
This item was published in
Chemometrics and Intelligent Laboratory Systems. 2006, vol. 80, p. 186-197
Elsevier
English Abstract
In a previous paper (part I), new strategy was used for raising antibodies against hapten (<300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising ...Read more >
In a previous paper (part I), new strategy was used for raising antibodies against hapten (<300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising antibodies against histamine, they were characterized and used for ELISA measurements. Optimal detection conditions were determined for the histamine quantification by ELISA method. The present study investigates the derivatization step of the histamine by chloroethylnitrosourea (CENU). Five factors (2 qualitative: nature of the solvent and nature of the antibodies and 3 quantitative: pH, % of solvent and time of derivatization) have been considered. Optimal reaction conditions were established by calculation of a validated model.Read less <
Italian Keywords
Experimental design
Box Behnken
Antibodies
Derivatization
Histamine quantification
Origin
Hal imported