The vineyard industry is a major economic sector in the Bordeaux region, which is looking for new ways to valorize its by-products. Stilbenoids a class of non-flavonoid polyphenols are a group of bioactive molecules found in plants and particularly abundant in grapevines, offer promising opportunities. This group includes the well-known resveratrol and its oligomers such as E-vitisin B (a resveratrol tetramer). E-Vitisin B also called r-viniferin has shown significant antimycotoxin activity, making its extraction highly valuable. Previous studies have identified grapevine roots as a rich source of E-vitisin B highlighting the potential to transform vine co-products into new plant-derived bioactives. Objective The aim of this work is to evaluate different eco-extraction methods for recovering vitisin B from grapevine roots. The processes will be compared in terms of vitisin B yield and selectivity. Methods Supercritical fluid extraction (SFE) was performed with home made setups on 4 and 30 g of ground vine roots. Effects of temperature, pressure, co-solvent composition and ratio with sc-CO2 were studied. Accelerated solvent extraction (ASE) was performed on Dionex ASE 350 system (Sunnyvale, California) at 100 bar on 5 g of plant powder mixed with sand. Number of cycles, solvent composition (EtOH / water), contact time and temperature were varied. E-Vitisin B was quantified through HPLC-DAD method. Results A full factorial 33 design of experiments conducted on ASE coupled with an optimization leads to a maximal yield of 22.3 g vitisin B/ kg dried roots. The major factor affecting the yield was the composition of the solvent. For SFE, a Box Behnken design of experiment leads to a maximal yield of 12.5 g/kg. The pressure was the main factor affecting the extraction. Selectivity of extraction for SFE was mainly determined by the co-solvent composition. So, both processes allowed for E-vitisin B extraction with quantities compatible with a high added-value use. Activity tests will be conducted to check the bioactivity of the extracts and the influence of the non-E-vitisin B compounds co-extracted.< Leer menos