Glucuronide metabolites of trans-ϵ-viniferin decrease triglycerides accumulation in an in vitro model of hepatic steatosis
Langue
EN
Article de revue
Ce document a été publié dans
Journal of physiology and biochemistry. 2024-07-31, vol. 80, n° 3, p. 685-696
Résumé en anglais
Trans-ε-viniferin, a resveratrol dimer found mainly in grapevine wood, has shown protective capacities against hepatic steatosis in vivo. Nevertheless, this compound is very poorly bioavailable. Thus, the aim of the present ...Lire la suite >
Trans-ε-viniferin, a resveratrol dimer found mainly in grapevine wood, has shown protective capacities against hepatic steatosis in vivo. Nevertheless, this compound is very poorly bioavailable. Thus, the aim of the present study is to determine the potential anti-steatotic properties of 1 and 10 µM of trans-ε-viniferin and its four glucuronide metabolites in AML-12 cells treated with palmitic acid as an in vitro model of hepatic steatosis. The effect of the molecules in cell viability and triglyceride accumulation, and the underlying mechanisms of action by Real-Time PCR and Western Blot were analysed, as well as the quantification of trans-ε-viniferin and the identified bioactive metabolite inside cells and their incubation media. Interestingly, we were able to determine the triglyceride-lowering property of one of the glucuronides (trans-ε-viniferin-2-glucuronide), which acts on de novo lipogenesis, fatty acid uptake and triglyceride assembly. The glucuronides of trans-ε-viniferin would therefore be partly responsible for the in vivo observed anti-steatotic properties of the parent compound. © The Author(s) 2024.< Réduire
Mots clés en anglais
Aml-12-Hepatocytes
Glucuronide Metabolites
Liver Steatosis
Resveratrol Dimer
Trans-Epsilon-Viniferin
Unités de recherche