BEAUMONT, Pauline; AMINTAS, Samuel; KRISA, Stéphanie; COURTOIS, Arnaud; RICHARD, Tristan; ESEBERRI, Itziar; PORTILLO, Maria P

doi:10.1007/s13105-024-01035-w

Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

AMINTAS, Samuel
BoRdeaux Institute in onCology [Inserm U1312 - BRIC]

KRISA, Stéphanie
Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

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Article de revue

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Journal of physiology and biochemistry. 2024-07-31, vol. 80, n° 3, p. 685-696

Resumen en inglés

Trans-ε-viniferin, a resveratrol dimer found mainly in grapevine wood, has shown protective capacities against hepatic steatosis in vivo. Nevertheless, this compound is very poorly bioavailable. Thus, the aim of the present study is to determine the potential anti-steatotic properties of 1 and 10 µM of trans-ε-viniferin and its four glucuronide metabolites in AML-12 cells treated with palmitic acid as an in vitro model of hepatic steatosis. The effect of the molecules in cell viability and triglyceride accumulation, and the underlying mechanisms of action by Real-Time PCR and Western Blot were analysed, as well as the quantification of trans-ε-viniferin and the identified bioactive metabolite inside cells and their incubation media. Interestingly, we were able to determine the triglyceride-lowering property of one of the glucuronides (trans-ε-viniferin-2-glucuronide), which acts on de novo lipogenesis, fatty acid uptake and triglyceride assembly. The glucuronides of trans-ε-viniferin would therefore be partly responsible for the in vivo observed anti-steatotic properties of the parent compound. © The Author(s) 2024.< Leer menos

Palabras clave en inglés

Aml-12-Hepatocytes

Glucuronide Metabolites

Liver Steatosis

Resveratrol Dimer

Trans-Epsilon-Viniferin

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https://oskar-bordeaux.fr/handle/20.500.12278/205893

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http://dx.doi.org/10.1007/s13105-024-01035-w

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Glucuronide metabolites of trans-ϵ-viniferin decrease triglycerides accumulation in an in vitro model of hepatic steatosis

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Glucuronide metabolites of trans-ϵ-viniferin decrease triglycerides accumulation in an in vitro model of hepatic steatosis

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Palabras clave en inglés

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DOI

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