Labeling of Membrane Complexes for Electron Microscopy
Language
en
Chapitre d'ouvrage
This item was published in
Membrane Protein Structure and Function Characterization. 2017-07-29, vol. 1635, p. 125-138
Humana Press
English Abstract
Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in ...Read more >
Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in difficult genetic manipulation and instability upon purification. Here, we present different labeling approaches that have been demonstrated successful in the structural characterization of large membrane complexes.Read less <
English Keywords
Antibody
Electron microscopy
Gold
Labeling
Membrane complexes
Negative stain
Tag