Labeling of Membrane Complexes for Electron Microscopy
Idioma
en
Chapitre d'ouvrage
Este ítem está publicado en
Membrane Protein Structure and Function Characterization. 2017-07-29, vol. 1635, p. 125-138
Humana Press
Resumen en inglés
Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in ...Leer más >
Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in difficult genetic manipulation and instability upon purification. Here, we present different labeling approaches that have been demonstrated successful in the structural characterization of large membrane complexes.< Leer menos
Palabras clave en inglés
Antibody
Electron microscopy
Gold
Labeling
Membrane complexes
Negative stain
Tag
Centros de investigación