Labeling of Membrane Complexes for Electron Microscopy
| dc.contributor.author | GUBELLINI, Francesca | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | FRONZES, Rémi | |
| dc.date.accessioned | 2023-06-28T11:24:51Z | |
| dc.date.available | 2023-06-28T11:24:51Z | |
| dc.date.issued | 2017-07-29 | |
| dc.identifier.isbn | 978-1-4939-7151-0 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/183227 | |
| dc.description.abstractEn | Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in difficult genetic manipulation and instability upon purification. Here, we present different labeling approaches that have been demonstrated successful in the structural characterization of large membrane complexes. | |
| dc.language.iso | en | |
| dc.publisher | Humana Press | |
| dc.publisher.location | New York, NY | |
| dc.source.title | Membrane Protein Structure and Function Characterization | en_US |
| dc.subject.en | Antibody | |
| dc.subject.en | Electron microscopy | |
| dc.subject.en | Gold | |
| dc.subject.en | Labeling | |
| dc.subject.en | Membrane complexes | |
| dc.subject.en | Negative stain | |
| dc.subject.en | Tag | |
| dc.subject.mesh | Bacterial Proteins | |
| dc.subject.mesh | Gold | |
| dc.subject.mesh | Membrane Proteins | |
| dc.subject.mesh | Microscopy, Electron | |
| dc.subject.mesh | Staining and Labeling | |
| dc.subject.mesh | Type IV Secretion Systems | |
| dc.subject.mesh | Type VI Secretion Systems | |
| dc.title.en | Labeling of Membrane Complexes for Electron Microscopy | |
| dc.type | Chapitre d'ouvrage | en_US |
| dc.identifier.doi | 10.1007/978-1-4939-7151-0_7 | |
| dc.subject.hal | Chimie/Cristallographie | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM] | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire | |
| dc.subject.hal | Informatique [cs]/Bio-informatique [q-bio.QM] | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie cellulaire | |
| bordeaux.page | 125-138 | |
| bordeaux.volume | 1635 | |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.institution | CNRS | |
| bordeaux.import.source | hal | |
| hal.identifier | pasteur-03263355 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Membrane%20Protein%20Structure%20and%20Function%20Characterization&rft.date=2017-07-29&rft.volume=1635&rft.spage=125-138&rft.epage=125-138&rft.au=GUBELLINI,%20Francesca&FRONZES,%20R%C3%A9mi&rft.isbn=978-1-4939-7151-0&rft.genre=unknown |
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