Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design
GEFFARD, M.
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
DOBRIJEVIC, M.
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Observatoire aquitain des sciences de l'univers [OASU]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
< Reduce
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Observatoire aquitain des sciences de l'univers [OASU]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Language
en
Article de revue
This item was published in
Chemometrics and Intelligent Laboratory Systems. 2006, vol. 80, p. 176-185
Elsevier
English Abstract
Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (<300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed ...Read more >
Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (<300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. The development of a quantitative method needs an optimization of both detection and derivatization step. Experimental design methodology has been applied to optimize the conditions of the detection of the derivatized histamine. Methods and results were closely related.Read less <
Spanish Keywords
Experimental design
Hybrid design
Antibodies
Derivatization
Histamine analysis
Origin
Hal imported