This study explored the extraction of antioxidant compounds from white wine lees using subcritical water extraction (SWE). First, SWE was compared to extraction using conventional solvents mixed with water: 50 % methanol, 50 % ethanol and 50 % acetonitrile. The antiradical activity of the extract increased to 12.8 mg TE/g of extract dry matter (DM) using conventional solvents and to 29.2 mg TE/g DM with SWE, compared to the antiradical activity of dried lees, which was 5.7 mg Trolox Equivalent/g of dry matter (mg TE/g DM). Second, SWE conditions were optimised to obtain lees extracts with a high antioxidant activity by applying a Doehlert design and response surface methodology (RSM). The extraction parameters modulated during RSM optimisation were extraction time (t, 15 – 60 min), temperature (T, 100 – 250 °C) and stirring speed (S, 100 – 1000 RPM). Antioxidant activity was assessed using DPPH and FRAP tests, as well as by measuring the Oxygen Consumption Rate (OCR) of lees extracts. Temperature was found to be the main parameter that had a significant effect on antioxidant activity. The results of the RSM showed the optimal conditions (temperature, duration and speed) for attaining maximum antioxidant activity to be: 240 °C, 15 min and 550 RPM respectively. The best-known antioxidant compounds in white wine lees, Total Polyphenol Content (TPC), Glutathione (GSH) and Total Sulfhydryl groups (TSH) were also quantified. Extracts with the highest antioxidant capacity had the highest TPC and TSH concentrations. In conclusion, this study showed (i) that subcritical water extraction is a green process that has potential as an alternative to using conventional solvents for extracting white wine lees, (ii) the chemical components involved in their antioxidant capacity, and (iii) the potential of the lees for oenological and other industrial applications.< Réduire