Proteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly
GUILLONNEAU, François
Institut Cochin [IC UM3 (UMR 8104 / U1016)]
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers [CRCI2NA ]
Institut Cochin [IC UM3 (UMR 8104 / U1016)]
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers [CRCI2NA ]
PARISSI, Vincent
Microbiologie Fondamentale et Pathogénicité [MFP]
Viral DNA Integration and Chromatin Dynamics Network [DyNAVir]
Microbiologie Fondamentale et Pathogénicité [MFP]
Viral DNA Integration and Chromatin Dynamics Network [DyNAVir]
MEERTENS, Laurent
Génomes, biologie cellulaire et thérapeutiques [GenCellDis (U944 / UMR7212)]
Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine ; ex- Institut Universitaire Hématologie-IUH) [IRSL]
Hopital Saint-Louis [AP-HP] [AP-HP]
< Reduce
Génomes, biologie cellulaire et thérapeutiques [GenCellDis (U944 / UMR7212)]
Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine ; ex- Institut Universitaire Hématologie-IUH) [IRSL]
Hopital Saint-Louis [AP-HP] [AP-HP]
Language
EN
Article de revue
This item was published in
Nature Communications. 2024-01-20, vol. 15, n° 1, p. 640
English Abstract
Considerable progress has been made in understanding the molecular host-virus battlefield during SARS-CoV-2 infection. Nevertheless, the assembly and egress of newly formed virions are less understood. To identify host ...Read more >
Considerable progress has been made in understanding the molecular host-virus battlefield during SARS-CoV-2 infection. Nevertheless, the assembly and egress of newly formed virions are less understood. To identify host proteins involved in viral morphogenesis, we characterize the proteome of SARS-CoV-2 virions produced from A549-ACE2 and Calu-3 cells, isolated via ultracentrifugation on sucrose cushion or by ACE-2 affinity capture. Bioinformatic analysis unveils 92 SARS-CoV-2 virion-associated host factors, providing a valuable resource to better understand the molecular environment of virion production. We reveal that G3BP1 and G3BP2 (G3BP1/2), two major stress granule nucleators, are embedded within virions and unexpectedly favor virion production. Furthermore, we show that G3BP1/2 participate in the formation of cytoplasmic membrane vesicles, that are likely virion assembly sites, consistent with a proviral role of G3BP1/2 in SARS-CoV-2 dissemination. Altogether, these findings provide new insights into host factors required for SARS-CoV-2 assembly with potential implications for future therapeutic targeting.Read less <
ANR Project
Les cellules présentatrices d'antigènes dans la maladie de COVID-19 à résolution monocellulaire - ANR-20-COV1-0001