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Proteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly

dc.rights.licenseopenen_US
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorMURIGNEUX, Emilie
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorSOFTIC, Laurent
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorAUBÉ, Corentin
hal.structure.identifierRadboud University [Nijmegen]
dc.contributor.authorGRANDI, Carmen
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorJUDITH, Delphine
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorBRUCE, Johanna
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorLE GALL, Morgane
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
hal.structure.identifierCentre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers [CRCI2NA]
dc.contributor.authorGUILLONNEAU, François
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorSCHMITT, Alain
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
hal.structure.identifierViral DNA Integration and Chromatin Dynamics Network [DyNAVir]
dc.contributor.authorPARISSI, Vincent
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorBERLIOZ-TORRENT, Clarisse
hal.structure.identifierGénomes, biologie cellulaire et thérapeutiques [GenCellDis (U944 / UMR7212)]
hal.structure.identifierInstitut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine ; ex- Institut Universitaire Hématologie-IUH) [IRSL]
hal.structure.identifierHopital Saint-Louis [AP-HP] [AP-HP]
dc.contributor.authorMEERTENS, Laurent
hal.structure.identifierRadboud University [Nijmegen]
dc.contributor.authorHANSEN, Maike
hal.structure.identifierInstitut Cochin [IC UM3 (UMR 8104 / U1016)]
dc.contributor.authorGALLOIS-MONTBRUN, Sarah
dc.date.accessioned2025-02-18T08:07:36Z
dc.date.available2025-02-18T08:07:36Z
dc.date.issued2024-01-20
dc.identifier.issn2041-1723en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/204995
dc.description.abstractEnConsiderable progress has been made in understanding the molecular host-virus battlefield during SARS-CoV-2 infection. Nevertheless, the assembly and egress of newly formed virions are less understood. To identify host proteins involved in viral morphogenesis, we characterize the proteome of SARS-CoV-2 virions produced from A549-ACE2 and Calu-3 cells, isolated via ultracentrifugation on sucrose cushion or by ACE-2 affinity capture. Bioinformatic analysis unveils 92 SARS-CoV-2 virion-associated host factors, providing a valuable resource to better understand the molecular environment of virion production. We reveal that G3BP1 and G3BP2 (G3BP1/2), two major stress granule nucleators, are embedded within virions and unexpectedly favor virion production. Furthermore, we show that G3BP1/2 participate in the formation of cytoplasmic membrane vesicles, that are likely virion assembly sites, consistent with a proviral role of G3BP1/2 in SARS-CoV-2 dissemination. Altogether, these findings provide new insights into host factors required for SARS-CoV-2 assembly with potential implications for future therapeutic targeting.
dc.description.sponsorshipLes cellules présentatrices d'antigènes dans la maladie de COVID-19 à résolution monocellulaire - ANR-20-COV1-0001en_US
dc.language.isoENen_US
dc.title.enProteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41467-024-44958-0en_US
dc.subject.halSciences du Vivant [q-bio]en_US
dc.identifier.pubmed38245532en_US
bordeaux.journalNature Communicationsen_US
bordeaux.page640en_US
bordeaux.volume15en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue1en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-04591662
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Communications&rft.date=2024-01-20&rft.volume=15&rft.issue=1&rft.spage=640&rft.epage=640&rft.eissn=2041-1723&rft.issn=2041-1723&rft.au=MURIGNEUX,%20Emilie&SOFTIC,%20Laurent&AUB%C3%89,%20Corentin&GRANDI,%20Carmen&JUDITH,%20Delphine&rft.genre=article


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