METOYER, Benjamin; RENOUF, Elodie; JOURDES, Michael; MERILLON, Jean-Michel; WAFFO TEGUO, Pierre

doi:10.1021/acs.jnatprod.3c00524

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METOYER, Benjamin
Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

RENOUF, Elodie
Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

JOURDES, Michael

Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

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Ce document a été publié dans

Journal of Natural Products. 2024-02-15

Résumé en anglais

Castanea sativa wood is a rich source of hydrolyzable tannins, known for their diverse bioactivities. To investigate these bioactive properties further, it is crucial to isolate and characterize hydrophilic compounds effectively. To address this issue, we developed a centrifugal partition chromatography (CPC) method and applied it to an aqueous C. sativa wood extract. We determined the partition coefficients (KD) of the six major compounds using four butanol-/water-based biphasic solvent systems. Initially, we utilized the n-butanol/propanol/water (3:1:4, v/v/v) systems for the first fractionation step. Subsequently, we employed the water/methyl tert-butyl ether/butanol/acetone (8:5:3:4, v/v/v/v) system to fractionate moderately and highly hydrophilic fractions. We calculated the KD values for major compounds of the most hydrophilic fractions using the butanol/ethanol/water (4:1:5, v/v/v) and butanol/isopropanol/water (2:1:3, v/v/v) systems. In total, we isolated 23 compounds through a combination of CPC, size exclusion chromatography, and preparative HPLC. Among these compounds, six have never been previously described. We characterized them by 1D and 2D NMR experiments and high-resolution mass spectroscopy acquisitions. © 2024 American Chemical Society and American Society of Pharmacognosy.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/199267

DOI

http://dx.doi.org/10.1021/acs.jnatprod.3c00524

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010

Unités de recherche

Oenologie - UMR 1366