Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy
SPINDLER, Susann
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
KÖNIG, Katharina
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
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Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
SPINDLER, Susann
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
KÖNIG, Katharina
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
PILIARIK, Marek
Max Planck Institute for the Science of Light
Institute of Photonics and Electronics of the Czech Academy of Sciences [UFE / CAS]
Max Planck Institute for the Science of Light
Institute of Photonics and Electronics of the Czech Academy of Sciences [UFE / CAS]
STEIN, Hannah E.
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
GARANGER, Elisabeth
Laboratoire de Chimie des Polymères Organiques [LCPO]
Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
LECOMMANDOUX, Sebastien
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
SANDOGHDAR, Vahid
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
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Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]
Language
en
Article de revue
This item was published in
Journal of Physics D: Applied Physics. 2016, vol. 49, n° 27, p. 274002
IOP Publishing
English Abstract
Microscopy based on the interferometric detection of light scattered from nanoparticles (iSCAT) was introduced in our laboratory more than a decade ago. In this work, we present various capabilities of iSCAT for biological ...Read more >
Microscopy based on the interferometric detection of light scattered from nanoparticles (iSCAT) was introduced in our laboratory more than a decade ago. In this work, we present various capabilities of iSCAT for biological studies by discussing a selection of our recent results. In particular, we show tracking of lipid molecules in supported lipid bilayers (SLBs), tracking of gold nanoparticles with diameters as small as 5 nm and at frame rates close to 1 MHz, 3D tracking of Tat peptide-coated nanoparticles on giant unilamellar vesicles (GUVs), imaging the formation of lipid bilayers, sensing single unlabelled proteins and tracking their motion under electric fields, as well as challenges of studying live cell membranes. These studies set the ground for future quantitative research on dynamic biophysical processes at the nanometer scale.Read less <
English Keywords
Electrophoresis
Lipid bilayers
Proteins
Electromagnetic wave scattering
Nanoparticles
Electric fields
Cytology
single-particle tracking
Interferometric detection
Molecules
Light scattering
Interferometry
protein detection
single protein electrophoresis
lipid diffusion
single molecule detection
Origin
Hal imported