SPINDLER, Susann; EHRIG, Jens; KÖNIG, Katharina; NOWAK, Tristan; PILIARIK, Marek; STEIN, Hannah E.; TAYLOR, Richard W.; GARANGER, Elisabeth; LECOMMANDOUX, Sebastien; ALVES, Isabel D.; SANDOGHDAR, Vahid

doi:10.1088/0022-3727/49/27/274002

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SPINDLER, Susann
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]

EHRIG, Jens
Max Planck Institute for the Science of Light
B CUBE-Ctr Mol Bioengn, TUD Dresden

KÖNIG, Katharina
Max Planck Institute for the Science of Light
Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU]

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Journal of Physics D: Applied Physics. 2016, vol. 49, n° 27, p. 274002

IOP Publishing

Résumé en anglais

Microscopy based on the interferometric detection of light scattered from nanoparticles (iSCAT) was introduced in our laboratory more than a decade ago. In this work, we present various capabilities of iSCAT for biological studies by discussing a selection of our recent results. In particular, we show tracking of lipid molecules in supported lipid bilayers (SLBs), tracking of gold nanoparticles with diameters as small as 5 nm and at frame rates close to 1 MHz, 3D tracking of Tat peptide-coated nanoparticles on giant unilamellar vesicles (GUVs), imaging the formation of lipid bilayers, sensing single unlabelled proteins and tracking their motion under electric fields, as well as challenges of studying live cell membranes. These studies set the ground for future quantitative research on dynamic biophysical processes at the nanometer scale.< Réduire

Mots clés en anglais

Electrophoresis

Lipid bilayers

Proteins

Electromagnetic wave scattering

Nanoparticles

Electric fields

Cytology

single-particle tracking

Interferometric detection

Molecules

Light scattering

Interferometry

protein detection

single protein electrophoresis

lipid diffusion

single molecule detection

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Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy

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