Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy
| dc.rights.license | open | |
| hal.structure.identifier | Max Planck Institute for the Science of Light | |
| hal.structure.identifier | Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU] | |
| dc.contributor.author | SPINDLER, Susann | |
| hal.structure.identifier | Max Planck Institute for the Science of Light | |
| hal.structure.identifier | B CUBE-Ctr Mol Bioengn, TUD Dresden | |
| dc.contributor.author | EHRIG, Jens | |
| hal.structure.identifier | Max Planck Institute for the Science of Light | |
| hal.structure.identifier | Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU] | |
| dc.contributor.author | KÖNIG, Katharina | |
| hal.structure.identifier | Optical Imaging Center Erlangen [OICE] | |
| dc.contributor.author | NOWAK, Tristan | |
| hal.structure.identifier | Max Planck Institute for the Science of Light | |
| hal.structure.identifier | Institute of Photonics and Electronics of the Czech Academy of Sciences [UFE / CAS] | |
| dc.contributor.author | PILIARIK, Marek | |
| hal.structure.identifier | Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU] | |
| dc.contributor.author | STEIN, Hannah E. | |
| hal.structure.identifier | Max Planck Institute for the Science of Light | |
| dc.contributor.author | TAYLOR, Richard W. | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | GARANGER, Elisabeth
IDREF: 089451740 | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | LECOMMANDOUX, Sebastien | |
| dc.contributor.author | ALVES, Isabel D. | |
| hal.structure.identifier | Max Planck Institute for the Science of Light | |
| hal.structure.identifier | Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg [FAU] | |
| dc.contributor.author | SANDOGHDAR, Vahid | |
| dc.date.accessioned | 2020 | |
| dc.date.available | 2020 | |
| dc.date.issued | 2016 | |
| dc.identifier.issn | 0022-3727 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/19686 | |
| dc.description.abstractEn | Microscopy based on the interferometric detection of light scattered from nanoparticles (iSCAT) was introduced in our laboratory more than a decade ago. In this work, we present various capabilities of iSCAT for biological studies by discussing a selection of our recent results. In particular, we show tracking of lipid molecules in supported lipid bilayers (SLBs), tracking of gold nanoparticles with diameters as small as 5 nm and at frame rates close to 1 MHz, 3D tracking of Tat peptide-coated nanoparticles on giant unilamellar vesicles (GUVs), imaging the formation of lipid bilayers, sensing single unlabelled proteins and tracking their motion under electric fields, as well as challenges of studying live cell membranes. These studies set the ground for future quantitative research on dynamic biophysical processes at the nanometer scale. | |
| dc.language.iso | en | |
| dc.publisher | IOP Publishing | |
| dc.subject.en | Electrophoresis | |
| dc.subject.en | Lipid bilayers | |
| dc.subject.en | Proteins | |
| dc.subject.en | Electromagnetic wave scattering | |
| dc.subject.en | Nanoparticles | |
| dc.subject.en | Electric fields | |
| dc.subject.en | Cytology | |
| dc.subject.en | single-particle tracking | |
| dc.subject.en | Interferometric detection | |
| dc.subject.en | Molecules | |
| dc.subject.en | Light scattering | |
| dc.subject.en | Interferometry | |
| dc.subject.en | protein detection | |
| dc.subject.en | single protein electrophoresis | |
| dc.subject.en | lipid diffusion | |
| dc.subject.en | single molecule detection | |
| dc.title.en | Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1088/0022-3727/49/27/274002 | |
| dc.subject.hal | Chimie/Chimie analytique | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Chimie-Physique [physics.chem-ph] | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Optique [physics.optics] | |
| bordeaux.journal | Journal of Physics D: Applied Physics | |
| bordeaux.page | 274002 | |
| bordeaux.volume | 49 | |
| bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
| bordeaux.issue | 27 | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-01372499 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-01372499v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Physics%20D:%20Applied%20Physics&rft.date=2016&rft.volume=49&rft.issue=27&rft.spage=274002&rft.epage=274002&rft.eissn=0022-3727&rft.issn=0022-3727&rft.au=SPINDLER,%20Susann&EHRIG,%20Jens&K%C3%96NIG,%20Katharina&NOWAK,%20Tristan&PILIARIK,%20Marek&rft.genre=article |
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