Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae
Language
en
Article de revue
This item was published in
Cell. 2011-02-04, vol. 144, n° 3, p. 402-413
Elsevier
English Abstract
The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. ...Read more >
The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin-and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin-and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.Read less <
English Keywords
SIGNAL-REGULATED KINASE
MEMBRANE TENSION
DEPENDENT ACTIVATION
ENDOTHELIAL-CELLS
IN-VITRO
PROTEIN
ENDOCYTOSIS
MUTATIONS
MUSCLE
GENE
ANR Project
Cellular responses to mechanical stress: interplay between membrane tension and membrane dynamics - ANR-06-BLAN-0211
Origin
Hal imported