SINHA, Bidisha; KOESTER, Darius; RUEZ, Richard; GONNORD, Pauline; BASTIANI, Michele; ABANKAWA, Daniel; STAN, Radu; BUTLER-BROWNE, Gillian; VEDIE, Benoît; JOHANNES, Ludger; MORONE, Nobuhiro; PARTON, Robert; RAPOSO, Graça; SENS, Pierre; LAMAZE, Christophe; NASSOY, Pierre

doi:10.1016/j.cell.2010.12.031

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

SINHA, Bidisha
Physico-Chimie-Curie [PCC]

KOESTER, Darius
Physico-Chimie-Curie [PCC]

RUEZ, Richard
Compartimentation et dynamique cellulaires [CDC]

Langue

Article de revue

Ce document a été publié dans

Cell. 2011-02-04, vol. 144, n° 3, p. 402-413

Elsevier

Résumé en anglais

The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin-and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin-and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.< Réduire

Mots clés en anglais

SIGNAL-REGULATED KINASE

MEMBRANE TENSION

DEPENDENT ACTIVATION

ENDOTHELIAL-CELLS

IN-VITRO

PROTEIN

ENDOCYTOSIS

MUTATIONS

MUSCLE

GENE

URI

https://oskar-bordeaux.fr/handle/20.500.12278/181151

DOI

http://dx.doi.org/10.1016/j.cell.2010.12.031

Project ANR

Cellular responses to mechanical stress: interplay between membrane tension and membrane dynamics - ANR-06-BLAN-0211

Origine

Importé de hal

Unités de recherche

Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298