MAVINGA, Marine; PALMIER, Mathilde; REMY, Murielle; JEANNIERE, Caroline; LENOIR, Solene; REY, Sylvie; SAINT-MARC, Martine; ALONSO, Florian; GENOT, Elisabeth; THEBAUD, Noelie; CHEVRET, Edith; MOURNETAS, Virginie; ROUSSEAU, Benoit; BOIZIAU, Claudine; BOEUF, Hélène

doi:10.3390/cells11244098

MAVINGA, Marine
Bioingénierie tissulaire [BIOTIS]

PALMIER, Mathilde

Bioingénierie tissulaire [BIOTIS]

REMY, Murielle
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Bioingénierie tissulaire [BIOTIS]

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Article de revue

Ce document a été publié dans

Cells. 2022-01, vol. 11, n° 24

Résumé en anglais

Tissue engineering strategies aim at characterizing and at optimizing the cellular component that is combined with biomaterials, for improved tissue regeneration. Here, we present the immunoMap of apical papilla, the native tissue from which SCAPs are derived. We characterized stem cell niches that correspond to a minority population of cells expressing Mesenchymal stromal/Stem Cell (CD90, CD105, CD146) and stemness (SSEA4 and CD49f) markers as well as endothelial cell markers (VWF, CD31). Based on the colocalization of TKS5 and cortactin markers, we detected migration-associated organelles, podosomes-like structures, in specific regions and, for the first time, in association with stem cell niches in normal tissue. From six healthy teenager volunteers, each with two teeth, we derived twelve cell banks, isolated and amplified under 21 or 3% O2. We confirmed a proliferative advantage of all banks when cultured under 3% versus 21% O2. Interestingly, telomerase activity was similar to that of the highly proliferative hiPSC cell line, but unrelated to O2 concentration. Finally, SCAPs embedded in a thixotropic hydrogel and implanted subcutaneously in immunodeficient mice were protected from cell death with a slightly greater advantage for cells preconditioned at 3% O2.< Réduire

Mots clés en anglais

apical papilla

engraftment

hypoxia

immunoMap

podosomes

SCAPs

thixotropic hydrogel

URI

https://oskar-bordeaux.fr/handle/20.500.12278/172048

DOI

http://dx.doi.org/10.3390/cells11244098

Unités de recherche

Bioingénierie Tissulaire (BioTis) - U1026

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The Journey of SCAPs (Stem Cells from Apical Papilla), from Their Native Tissue to Grafting: Impact of Oxygen Concentration

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Résumé en anglais

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