The Journey of SCAPs (Stem Cells from Apical Papilla), from Their Native Tissue to Grafting: Impact of Oxygen Concentration
REMY, Murielle
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Bioingénierie tissulaire [BIOTIS]
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Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Bioingénierie tissulaire [BIOTIS]
REMY, Murielle
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Bioingénierie tissulaire [BIOTIS]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Bioingénierie tissulaire [BIOTIS]
GENOT, Elisabeth
Bioingénierie tissulaire [BIOTIS]
Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
< Leer menos
Bioingénierie tissulaire [BIOTIS]
Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
Idioma
EN
Article de revue
Este ítem está publicado en
Cells. 2022-01, vol. 11, n° 24
Resumen en inglés
Tissue engineering strategies aim at characterizing and at optimizing the cellular component that is combined with biomaterials, for improved tissue regeneration. Here, we present the immunoMap of apical papilla, the native ...Leer más >
Tissue engineering strategies aim at characterizing and at optimizing the cellular component that is combined with biomaterials, for improved tissue regeneration. Here, we present the immunoMap of apical papilla, the native tissue from which SCAPs are derived. We characterized stem cell niches that correspond to a minority population of cells expressing Mesenchymal stromal/Stem Cell (CD90, CD105, CD146) and stemness (SSEA4 and CD49f) markers as well as endothelial cell markers (VWF, CD31). Based on the colocalization of TKS5 and cortactin markers, we detected migration-associated organelles, podosomes-like structures, in specific regions and, for the first time, in association with stem cell niches in normal tissue. From six healthy teenager volunteers, each with two teeth, we derived twelve cell banks, isolated and amplified under 21 or 3% O2. We confirmed a proliferative advantage of all banks when cultured under 3% versus 21% O2. Interestingly, telomerase activity was similar to that of the highly proliferative hiPSC cell line, but unrelated to O2 concentration. Finally, SCAPs embedded in a thixotropic hydrogel and implanted subcutaneously in immunodeficient mice were protected from cell death with a slightly greater advantage for cells preconditioned at 3% O2.< Leer menos
Palabras clave en inglés
apical papilla
engraftment
hypoxia
immunoMap
podosomes
SCAPs
thixotropic hydrogel
Centros de investigación