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The Journey of SCAPs (Stem Cells from Apical Papilla), from Their Native Tissue to Grafting: Impact of Oxygen Concentration

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorMAVINGA, Marine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorPALMIER, Mathilde
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREMY, Murielle
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorJEANNIERE, Caroline
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLENOIR, Solene
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREY, Sylvie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSAINT-MARC, Martine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorALONSO, Florian
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorGENOT, Elisabeth
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHEBAUD, Noelie
hal.structure.identifierBoRdeaux Institute in onCology [Inserm U1312 - BRIC]
dc.contributor.authorCHEVRET, Edith
dc.contributor.authorMOURNETAS, Virginie
dc.contributor.authorROUSSEAU, Benoit
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOIZIAU, Claudine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOEUF, Hélène
ORCID: 0000-0002-3006-8773
IDREF: 03205453X
dc.date.accessioned2023-02-22T08:30:59Z
dc.date.available2023-02-22T08:30:59Z
dc.date.issued2022-01
dc.identifier.issn2073-4409en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/172048
dc.description.abstractEnTissue engineering strategies aim at characterizing and at optimizing the cellular component that is combined with biomaterials, for improved tissue regeneration. Here, we present the immunoMap of apical papilla, the native tissue from which SCAPs are derived. We characterized stem cell niches that correspond to a minority population of cells expressing Mesenchymal stromal/Stem Cell (CD90, CD105, CD146) and stemness (SSEA4 and CD49f) markers as well as endothelial cell markers (VWF, CD31). Based on the colocalization of TKS5 and cortactin markers, we detected migration-associated organelles, podosomes-like structures, in specific regions and, for the first time, in association with stem cell niches in normal tissue. From six healthy teenager volunteers, each with two teeth, we derived twelve cell banks, isolated and amplified under 21 or 3% O2. We confirmed a proliferative advantage of all banks when cultured under 3% versus 21% O2. Interestingly, telomerase activity was similar to that of the highly proliferative hiPSC cell line, but unrelated to O2 concentration. Finally, SCAPs embedded in a thixotropic hydrogel and implanted subcutaneously in immunodeficient mice were protected from cell death with a slightly greater advantage for cells preconditioned at 3% O2.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enapical papilla
dc.subject.enengraftment
dc.subject.enhypoxia
dc.subject.enimmunoMap
dc.subject.enpodosomes
dc.subject.enSCAPs
dc.subject.enthixotropic hydrogel
dc.title.enThe Journey of SCAPs (Stem Cells from Apical Papilla), from Their Native Tissue to Grafting: Impact of Oxygen Concentration
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/cells11244098en_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
bordeaux.journalCellsen_US
bordeaux.volume11en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026en_US
bordeaux.issue24en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04000034
hal.version1
hal.date.transferred2023-02-22T08:31:09Z
hal.exporttrue
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.date=2022-01&rft.volume=11&rft.issue=24&rft.eissn=2073-4409&rft.issn=2073-4409&rft.au=MAVINGA,%20Marine&PALMIER,%20Mathilde&REMY,%20Murielle&JEANNIERE,%20Caroline&LENOIR,%20Solene&rft.genre=article


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