BARCHI, Yasma; PHILIPPE, Cécile; CHAÏB, Amel; OVIEDO-HERNANDEZ, Florencia; CLAISSE, Olivier; LE MARREC, Claire

doi:10.3390/v15010015

Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

PHILIPPE, Cécile
Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

CHAÏB, Amel
Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

Langue

Article de revue

Ce document a été publié dans

Viruses. 2022-12-20, vol. 15, n° 1, p. 15

Résumé en anglais

The Oenococcus genus comprises four recognized species, and members have been found in different types of beverages, including wine, kefir, cider and kombucha. In this work, we implemented two complementary strategies to assess whether oenococcal hosts of different species and habitats were connected through their bacteriophages. First, we investigated the diversity of CRISPR-Cas systems using a genome-mining approach, and CRISPR-endowed strains were identified in three species. A census of the spacers from the four identified CRISPR-Cas loci showed that each spacer space was mostly dominated by species-specific sequences. Yet, we characterized a limited records of potentially recent and also ancient infections between O. kitaharae and O. sicerae and phages of O. oeni, suggesting that some related phages have interacted in diverse ways with their Oenococcus hosts over evolutionary time. Second, phage-host interaction analyses were performed experimentally with a diversified panel of phages and strains. None of the tested phages could infect strains across the species barrier. Yet, some infections occurred between phages and hosts from distinct beverages in the O. oeni species.< Réduire

Mots clés en anglais

Fermented beverage

Oenococcus

Bacteriophage

CRISPR

Spacers

Host spectra

URI

https://oskar-bordeaux.fr/handle/20.500.12278/171899

DOI

http://dx.doi.org/10.3390/v15010015

Lien vers les données de la recherche

https://www.ncbi.nlm.nih.gov/genbank/
https://mdpi-res.com/d_attachment/viruses/viruses-15-00015/article_deploy/viruses-15-00015-s001.zip?version=1672055606

Project ANR

Simuler des Evenements Climatiques Rares - ANR-20-CE01-0008

Unités de recherche

Oenologie - UMR 1366

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Phage Encounters Recorded in CRISPR Arrays in the Genus Oenococcus