Study of the interaction of β-cyclodextrin with phospholipid monolayers by surface pressure measurements and fluorescence microscopy
Language
en
Article de revue
This item was published in
Journal of Colloid and Interface Science. 2008-06-01, vol. 322, n° 1, p. 73-78
Elsevier
English Abstract
The interaction of β-cyclodextrin (β-CD) with different lipids has been studied, using Langmuir monolayers kept at constant surface pressure or constant spreading surface. Results show that β-CD, injected beneath the ...Read more >
The interaction of β-cyclodextrin (β-CD) with different lipids has been studied, using Langmuir monolayers kept at constant surface pressure or constant spreading surface. Results show that β-CD, injected beneath the monolayer, is able to desorb unsaturated palmitoyloleoylphosphatidylcholine (POPC) and sphingomyelin (SM) under specific experimental conditions. In this last case, SM monolayers, labeled with the fluorescent NBD-PC probe, were also observed by fluorescence microscopy, before and after β-CD injection. Images show that SM monolayers are more homogeneous after β-CD injection, because of the lipid desorption. At last, it seems that lipid desorption occurs only in a restricted surface pressure range, depending on the lipid.Read less <
English Keywords
β-Cyclodextrin
Phospholipid
Monolayer
Desorption
Surface pressure measurements
Fluorescence microscopy
Origin
Hal imported