Protein Extraction from Woody Plants
Language
en
Chapitre d'ouvrage
This item was published in
Plant Proteomics: Methods and Protocols. 2007, vol. 355
Humana Press
English Abstract
In this chapter we present a protocol for total protein extraction optimized for woodforming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, ...Read more >
In this chapter we present a protocol for total protein extraction optimized for woodforming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php.Read less <
Keywords
TOTAL PROTEIN EXTRACTION
English Keywords
TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS
WOOD
TREE
ESPECE A FEUILLES LARGES
METHODE TCA-ACETONE
ELECTROPHORESE EN GEL DE POLYACRYLAMIDE
Origin
Hal imported