Protein Extraction from Woody Plants
Langue
en
Chapitre d'ouvrage
Ce document a été publié dans
Plant Proteomics: Methods and Protocols. 2007, vol. 355
Humana Press
Résumé en anglais
In this chapter we present a protocol for total protein extraction optimized for woodforming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, ...Lire la suite >
In this chapter we present a protocol for total protein extraction optimized for woodforming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php.< Réduire
Mots clés
TOTAL PROTEIN EXTRACTION
Mots clés en anglais
TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS
WOOD
TREE
ESPECE A FEUILLES LARGES
METHODE TCA-ACETONE
ELECTROPHORESE EN GEL DE POLYACRYLAMIDE
Origine
Importé de halUnités de recherche