Protein Extraction from Woody Plants
Idioma
en
Chapitre d'ouvrage
Este ítem está publicado en
Plant Proteomics: Methods and Protocols. 2007, vol. 355
Humana Press
Resumen en inglés
In this chapter we present a protocol for total protein extraction optimized for woodforming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, ...Leer más >
In this chapter we present a protocol for total protein extraction optimized for woodforming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php.< Leer menos
Palabras clave
TOTAL PROTEIN EXTRACTION
Palabras clave en inglés
TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS
WOOD
TREE
ESPECE A FEUILLES LARGES
METHODE TCA-ACETONE
ELECTROPHORESE EN GEL DE POLYACRYLAMIDE
Orígen
Importado de HalCentros de investigación