MUGGIOLU, Giovanna; POMORSKI, Michal; CLAVERIE, Gérard; BERTHET, Guillaume; MER-CALFATI, Christine; SAADA, Samuel; DEVÈS, Guillaume; SIMON, Marina; SEZNEC, Herve; BARBERET, Philippe

doi:10.1038/srep41764

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MUGGIOLU, Giovanna
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

POMORSKI, Michal
Laboratoire d'Intégration des Systèmes et des Technologies [LIST (CEA)]

CLAVERIE, Gérard
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

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Scientific Reports. 2017, vol. 7, p. 41764

Nature Publishing Group

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As well as being a significant source of environmental radiation exposure, α-particles are increasinglyconsidered for use in targeted radiation therapy. A better understanding of α-particle induced damageat the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focusedα-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limitedrange, α-particles detection, delivery, and follow-up observations of radiation-induced damage remaindifficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane thatallows reliable single α-particles detection and single cell irradiation with negligible beam scattering.The radiation-induced responses of single 3MeV α-particles delivered with focused microbeam arevisualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8protein at DNA damaged sites.< Réduire

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Single $\alpha$-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites

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