Single $\alpha$-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites
Language
en
Article de revue
This item was published in
Scientific Reports. 2017, vol. 7, p. 41764
Nature Publishing Group
English Abstract
As well as being a significant source of environmental radiation exposure, α-particles are increasinglyconsidered for use in targeted radiation therapy. A better understanding of α-particle induced damageat the DNA scale ...Read more >
As well as being a significant source of environmental radiation exposure, α-particles are increasinglyconsidered for use in targeted radiation therapy. A better understanding of α-particle induced damageat the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focusedα-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limitedrange, α-particles detection, delivery, and follow-up observations of radiation-induced damage remaindifficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane thatallows reliable single α-particles detection and single cell irradiation with negligible beam scattering.The radiation-induced responses of single 3MeV α-particles delivered with focused microbeam arevisualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8protein at DNA damaged sites.Read less <
Origin
Hal imported