Single $\alpha$-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites
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en
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Este ítem está publicado en
Scientific Reports. 2017, vol. 7, p. 41764
Nature Publishing Group
Resumen en inglés
As well as being a significant source of environmental radiation exposure, α-particles are increasinglyconsidered for use in targeted radiation therapy. A better understanding of α-particle induced damageat the DNA scale ...Leer más >
As well as being a significant source of environmental radiation exposure, α-particles are increasinglyconsidered for use in targeted radiation therapy. A better understanding of α-particle induced damageat the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focusedα-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limitedrange, α-particles detection, delivery, and follow-up observations of radiation-induced damage remaindifficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane thatallows reliable single α-particles detection and single cell irradiation with negligible beam scattering.The radiation-induced responses of single 3MeV α-particles delivered with focused microbeam arevisualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8protein at DNA damaged sites.< Leer menos
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