PHILIPPE, Cecile; KRUPOVIC, Mart; JAOMANJAKA, Fety; CLAISSE, Olivier; PETREL, Melina; LE MARREC, Claire

doi:10.3390/v10010039

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

PHILIPPE, Cecile
Unité de Recherche Oenologie [Villenave d'Ornon] [OENO]

KRUPOVIC, Mart

JAOMANJAKA, Fety
Unité de Recherche Oenologie [Villenave d'Ornon] [OENO]

Langue

Article de revue

Ce document a été publié dans

Viruses. 2018, vol. 10, n° 1, p. 1-16

Résumé en anglais

The Gluconobacter phage GC1 is a novel member of the Tectiviridae family isolated from a juice sample collected during dry white wine making. The bacteriophage infects Gluconobacter cerinus, an acetic acid bacterium which represents a spoilage microorganism during wine making, mainly because it is able to produce ethyl alcohol and transform it into acetic acid. Transmission electron microscopy revealed tail-less icosahedral particles with a diameter of similar to 78 nm. The linear double-stranded DNA genome of GC1 (16,523 base pairs) contains terminal inverted repeats and carries 36 open reading frames, only a handful of which could be functionally annotated. These encode for the key proteins involved in DNA replication (protein-primed family B DNA polymerase) as well as in virion structure and assembly (major capsid protein, genome packaging ATPase (adenosine triphosphatase) and several minor capsid proteins). GC1 is the first tectivirus infecting an alphaproteobacterial host and is thus far the only temperate tectivirus of gram-negative bacteria. Based on distinctive sequence and life-style features, we propose that GC1 represents a new genus within the Tectiviridae, which we tentatively named Gammatectivirus. Furthermore, GC1 helps to bridge the gap in the sequence space between alphatectiviruses and betatectiviruses.< Réduire

Mots clés

Gluconobacter

Vin blanc sec

Vitis Vinifera

Lutte contre les bactériophages

Acide acétique

Mots clés en anglais

Bacteriophage

Tectivirus

Wine Making

Must

Acetic Acid Bacteria

Gluconobacter Cerinus