Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design
GEFFARD, M.
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
Laboratoire de physique des interactions ondes matières [LPIOM]
Laboratoire de l'intégration, du matériau au système [IMS]
DOBRIJEVIC, M.
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Observatoire aquitain des sciences de l'univers [OASU]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
< Réduire
Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
Observatoire aquitain des sciences de l'univers [OASU]
Laboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
Langue
en
Article de revue
Ce document a été publié dans
Chemometrics and Intelligent Laboratory Systems. 2006, vol. 80, p. 176-185
Elsevier
Résumé en anglais
Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (<300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed ...Lire la suite >
Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (<300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. The development of a quantitative method needs an optimization of both detection and derivatization step. Experimental design methodology has been applied to optimize the conditions of the detection of the derivatized histamine. Methods and results were closely related.< Réduire
Mots clés en espagnol
Experimental design
Hybrid design
Antibodies
Derivatization
Histamine analysis
Origine
Importé de halUnités de recherche