BURE, Corinne; LE SENECHAL, Caroline; MACIAS, Luis; TOKARSKI, Caroline; VILAIN, Sebastien; BRODBELT, Jennifer S.

doi:10.1021/acs.analchem.0c05069

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

BURE, Corinne
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

LE SENECHAL, Caroline
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

MACIAS, Luis

Langue

Article de revue

Ce document a été publié dans

Analytical Chemistry. 2021, vol. 93, n° 9, p. 4255-4262

Résumé en anglais

Lipopolysaccharides (LPS) constitute the outermost layer of Gram-negative bacteria and consequently play an important role in bacterial infections. In order to address public health issues posed by Gram-negative bacteria, it is necessary to elucidate the structure of the molecular actors at the forefront of infections. LPS virulence and toxicity are partially modulated by lipid A, a hydrophobic saccharolipid that anchors LPS to the bacterial outer membrane. Understanding the lipid A structure is inherently intertwined with understanding its role as an endotoxin. Accordingly, several successful strategies incorporating tandem mass spectrometry have been applied toward the structural analysis of lipid A. Herein, a shotgun HCD strategy was applied toward the characterization of the lipid A profile of Pseudomonas aeruginosa PAO1. This analysis was enhanced by the development of an LC-MS/MS approach to eliminate isomeric signals in the MS/MS spectra that confounded characterization. Importantly, combining reverse phase chromatography with HCD and ultraviolet photodissociation analyses of the lipid A profile revealed the presence of previously unreported lipid A acyl chain positional isomers. Altogether, these strategies provide the most in-depth structural and molecular characterization of PAO1 lipid A to date.< Réduire

Mots clés en anglais

Lipids

Mass spectrometry

Ions

Chemical structure

Acyls

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Characterization of Isomers of Lipid A from Pseudomonas aeruginosa PAO1 by Liquid Chromatography with Tandem Mass Spectrometry with Higher-Energy Collisional Dissociation and Ultraviolet Photodissociation

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Lien vers les données de la recherche

Unités de recherche