Characterization of Isomers of Lipid A from Pseudomonas aeruginosa PAO1 by Liquid Chromatography with Tandem Mass Spectrometry with Higher-Energy Collisional Dissociation and Ultraviolet Photodissociation
dc.rights.license | open | en_US |
hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | BURE, Corinne | |
hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | LE SENECHAL, Caroline | |
dc.contributor.author | MACIAS, Luis | |
hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | TOKARSKI, Caroline | |
hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | VILAIN, Sebastien | |
dc.contributor.author | BRODBELT, Jennifer S. | |
dc.date.accessioned | 2021-07-01T09:53:09Z | |
dc.date.available | 2021-07-01T09:53:09Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0003-2700 | en_US |
dc.identifier.other | https://pubs.acs.org/doi/10.1021/acs.analchem.0c05069?goto=supporting-info | en_US |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/94954 | |
dc.description.abstractEn | Lipopolysaccharides (LPS) constitute the outermost layer of Gram-negative bacteria and consequently play an important role in bacterial infections. In order to address public health issues posed by Gram-negative bacteria, it is necessary to elucidate the structure of the molecular actors at the forefront of infections. LPS virulence and toxicity are partially modulated by lipid A, a hydrophobic saccharolipid that anchors LPS to the bacterial outer membrane. Understanding the lipid A structure is inherently intertwined with understanding its role as an endotoxin. Accordingly, several successful strategies incorporating tandem mass spectrometry have been applied toward the structural analysis of lipid A. Herein, a shotgun HCD strategy was applied toward the characterization of the lipid A profile of Pseudomonas aeruginosa PAO1. This analysis was enhanced by the development of an LC-MS/MS approach to eliminate isomeric signals in the MS/MS spectra that confounded characterization. Importantly, combining reverse phase chromatography with HCD and ultraviolet photodissociation analyses of the lipid A profile revealed the presence of previously unreported lipid A acyl chain positional isomers. Altogether, these strategies provide the most in-depth structural and molecular characterization of PAO1 lipid A to date. | |
dc.language.iso | EN | en_US |
dc.subject.en | Lipids | |
dc.subject.en | Mass spectrometry | |
dc.subject.en | Ions | |
dc.subject.en | Chemical structure | |
dc.subject.en | Acyls | |
dc.title.en | Characterization of Isomers of Lipid A from Pseudomonas aeruginosa PAO1 by Liquid Chromatography with Tandem Mass Spectrometry with Higher-Energy Collisional Dissociation and Ultraviolet Photodissociation | |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.1021/acs.analchem.0c05069 | en_US |
dc.subject.hal | Chimie/Matériaux | en_US |
bordeaux.journal | Analytical Chemistry | en_US |
bordeaux.page | 4255-4262 | en_US |
bordeaux.volume | 93 | en_US |
bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | en_US |
bordeaux.issue | 9 | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.institution | Bordeaux INP | en_US |
bordeaux.institution | CNRS | en_US |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
hal.identifier | hal-03275570 | |
hal.version | 1 | |
hal.date.transferred | 2021-07-01T09:53:13Z | |
hal.export | true | |
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