BEAUMONT, Pauline; COURTOIS, Arnaud; RICHARD, Tristan; KRISA, Stephanie; FAURE, Chrystel

doi:10.3390/pharmaceutics13040566

Unité de Recherche Oenologie [Villenave d'Ornon] [OENO]

COURTOIS, Arnaud

Unité de Recherche Oenologie [Villenave d'Ornon] [OENO]

RICHARD, Tristan

Unité de Recherche Oenologie [Villenave d'Ornon] [OENO]

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Article de revue

Ce document a été publié dans

Pharmaceutics. 2021, vol. 13, n° 4, p. 566

Résumé en anglais

Onion-type multi-lamellar liposomes (MLLs), composed of a mixture of phosphatidylcholine and Tween 80, were analyzed for their ability to encapsulate epsilon-Viniferin (epsilon Vin), a resveratrol dimer. Their encapsulation efficiency (EE) was measured by UV-VIS spectroscopy using three different separation methods-ultracentrifugation, size exclusion chromatography, and a more original and advantageous one, based on adsorption filtration. The adsorption filtration method consists indeed of using syringe filters to retain the molecule of interest, and not the liposomes as usually performed. The process is rapid (less than 10 min), easy to handle, and inexpensive in terms of sample amount (around 2 mg of liposomes) and equipment (one syringe filter is required). Whatever the separation method, a similar EE value was determined, validating the proposed method. A total of 80% +/- 4% of epsilon Vin was found to be encapsulated leading to a 6.1% payload, roughly twice those reported for resveratrol-loaded liposomes. Finally, the release kinetics of epsilon Vin from MLLs was followed for a 77 day period, demonstrating a slow release of the polyphenol.< Réduire

Mots clés en anglais

encapsulation efficiency

ε-Viniferin

multi-lamellar liposomes

adsorption filtration

polyphenol

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https://oskar-bordeaux.fr/handle/20.500.12278/94951

DOI

http://dx.doi.org/10.3390/pharmaceutics13040566

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https://www.mdpi.com/1999-4923/13/4/566/s1

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Encapsulation of epsilon-Viniferin into Multi-Lamellar Liposomes: Development of a Rapid, Easy and Cost-Efficient Separation Method to Determine the Encapsulation Efficiency

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Encapsulation of epsilon-Viniferin into Multi-Lamellar Liposomes: Development of a Rapid, Easy and Cost-Efficient Separation Method to Determine the Encapsulation Efficiency

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Résumé en anglais

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