A Unique Triculture Model to Study Osteoblasts, Osteoclasts, and Endothelial Cells
Idioma
en
Article de revue
Este ítem está publicado en
Tissue Engineering Part C: Methods. 2019-07, vol. 25, n° 7, p. 421-432
Mary Ann Liebert
Resumen en inglés
In this article, we first developed a new medium to culture together primary human osteoblastic, osteoclastic, and endothelial cells (ECs) chosen to represent the three major bone cell tissues. Indeed, no study has been ...Leer más >
In this article, we first developed a new medium to culture together primary human osteoblastic, osteoclastic, and endothelial cells (ECs) chosen to represent the three major bone cell tissues. Indeed, no study has been conducted on primary human cells and on the phenotype/activity retention of these three primary human cell types. Thus, we established an original triculture model with osteoblastic, osteoclastic, and ECs, where not only both cell phenotype and cell activity were maintained but also cell culture homeostasis. These promising results will permit further investigations to create in vitro conditions to mimic the bone microenvironment and analyze cell interactions in ex vivo studies.< Leer menos
Palabras clave en inglés
cell phenotype
culture medium
human primary cells
triculture model.
Orígen
Importado de HalCentros de investigación