A Unique Triculture Model to Study Osteoblasts, Osteoclasts, and Endothelial Cells
GREMARE, Agathe
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
AUSSEL, Audrey
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
See more >
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
GREMARE, Agathe
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
AUSSEL, Audrey
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
THÉBAUD, Noelie
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
< Reduce
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
Language
en
Article de revue
This item was published in
Tissue Engineering Part C: Methods. 2019-07, vol. 25, n° 7, p. 421-432
Mary Ann Liebert
English Abstract
In this article, we first developed a new medium to culture together primary human osteoblastic, osteoclastic, and endothelial cells (ECs) chosen to represent the three major bone cell tissues. Indeed, no study has been ...Read more >
In this article, we first developed a new medium to culture together primary human osteoblastic, osteoclastic, and endothelial cells (ECs) chosen to represent the three major bone cell tissues. Indeed, no study has been conducted on primary human cells and on the phenotype/activity retention of these three primary human cell types. Thus, we established an original triculture model with osteoblastic, osteoclastic, and ECs, where not only both cell phenotype and cell activity were maintained but also cell culture homeostasis. These promising results will permit further investigations to create in vitro conditions to mimic the bone microenvironment and analyze cell interactions in ex vivo studies.Read less <
English Keywords
cell phenotype
culture medium
human primary cells
triculture model.
Origin
Hal importedCollections