WADEESIRISAK, Kanthida; CASTANO, Sabine; VAYSSE, Laurent; BONFILS, Frederic; PERUCH, Frederic; RATTANAPORN, Kittipong; LIENGPRAYOON, Siriluck; LECOMTE, Sophie; BOTTIER, Celine

doi:10.1016/j.bbrc.2023.08.062

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WADEESIRISAK, Kanthida

CASTANO, Sabine

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

VAYSSE, Laurent

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Biochemical and Biophysical Research Communications. 2023, vol. 679, p. 205-214

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According to the fatty acid and headgroup compositions of the phospholipids (PL) from Hevea brasiliensis latex, three synthetic PL were selected (i.e. POPA: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate POPC: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and POPG: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol) to investigate the effect of PL headgroup on the interactions with two major proteins of Hevea latex, i.e. Rubber Elongation Factor (REF1) and Small Rubber Particle Protein (SRPP1). Protein/lipid interactions were screened using two models (lipid vesicles in solution or lipid monolayers at air/liquid interface). Calcein leakage, surface pressure, ellipsometry, microscopy and spectroscopy revealed that both REF1 and SRPP1 displayed stronger interactions with anionic POPA and POPG, as compared to zwitterionic POPC. A particular behavior of REF1 was observed when interacting with POPA monolayers (i.e. aggregation + modification of secondary structure from α-helices to β-sheets, characteristic of its amyloid aggregated form), which might be involved in the irreversible coagulation mechanism of Hevea rubber particles.< Réduire

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Hevea brasiliensis

rubber particle proteins

rubber elongation factor

small rubber particle protein

anionic phospholipids

latex coagulation

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Interactions of REF1 and SRPP1 rubber particle proteins from Hevea brasiliensis with synthetic phospholipids: Effect of charge and size of lipid headgroup

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