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Interactions of REF1 and SRPP1 rubber particle proteins from Hevea brasiliensis with synthetic phospholipids: Effect of charge and size of lipid headgroup

dc.rights.licenseopenen_US
dc.contributor.authorWADEESIRISAK, Kanthida
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCASTANO, Sabine
dc.contributor.authorVAYSSE, Laurent
dc.contributor.authorBONFILS, Frederic
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorPERUCH, Frederic
dc.contributor.authorRATTANAPORN, Kittipong
dc.contributor.authorLIENGPRAYOON, Siriluck
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLECOMTE, Sophie
dc.contributor.authorBOTTIER, Celine
dc.date.accessioned2024-05-03T08:06:41Z
dc.date.available2024-05-03T08:06:41Z
dc.date.issued2023
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199589
dc.description.abstractEnAccording to the fatty acid and headgroup compositions of the phospholipids (PL) from Hevea brasiliensis latex, three synthetic PL were selected (i.e. POPA: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate POPC: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and POPG: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol) to investigate the effect of PL headgroup on the interactions with two major proteins of Hevea latex, i.e. Rubber Elongation Factor (REF1) and Small Rubber Particle Protein (SRPP1). Protein/lipid interactions were screened using two models (lipid vesicles in solution or lipid monolayers at air/liquid interface). Calcein leakage, surface pressure, ellipsometry, microscopy and spectroscopy revealed that both REF1 and SRPP1 displayed stronger interactions with anionic POPA and POPG, as compared to zwitterionic POPC. A particular behavior of REF1 was observed when interacting with POPA monolayers (i.e. aggregation + modification of secondary structure from α-helices to β-sheets, characteristic of its amyloid aggregated form), which might be involved in the irreversible coagulation mechanism of Hevea rubber particles.
dc.language.isoENen_US
dc.subject.enHevea brasiliensis
dc.subject.enrubber particle proteins
dc.subject.enrubber elongation factor
dc.subject.ensmall rubber particle protein
dc.subject.enanionic phospholipids
dc.subject.enlatex coagulation
dc.title.enInteractions of REF1 and SRPP1 rubber particle proteins from Hevea brasiliensis with synthetic phospholipids: Effect of charge and size of lipid headgroup
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.bbrc.2023.08.062en_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biophysiqueen_US
bordeaux.journalBiochemical and Biophysical Research Communicationsen_US
bordeaux.page205-214en_US
bordeaux.volume679en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-04273044
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biochemical%20and%20Biophysical%20Research%20Communications&rft.date=2023&rft.volume=679&rft.spage=205-214&rft.epage=205-214&rft.eissn=0006-291X&rft.issn=0006-291X&rft.au=WADEESIRISAK,%20Kanthida&CASTANO,%20Sabine&VAYSSE,%20Laurent&BONFILS,%20Frederic&PERUCH,%20Frederic&rft.genre=article


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