Membrane plasticity induced by myo-inositol derived archaeal lipids: chemical synthesis and biophysical characterization
GRELARD, Axelle
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
DUFOURC, Erick
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Institut Européen de Chimie et Biologie [IECB]
< Reduce
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Institut Européen de Chimie et Biologie [IECB]
Language
EN
Article de revue
This item was published in
Physical Chemistry Chemical Physics. 2023, vol. 25, n° 24, p. 16273 - 16287
English Abstract
Archaeal membrane lipids have specific structures that allow Archaea to withstand extreme conditions of temperature and pressure. In order to understand the molecular parameters that govern such resistance, the synthesis ...Read more >
Archaeal membrane lipids have specific structures that allow Archaea to withstand extreme conditions of temperature and pressure. In order to understand the molecular parameters that govern such resistance, the synthesis of 1,2-di-O-phytanyl-sn-glycero-3-phosphoinositol (DoPhPI), an archaeal lipid derived from myo-inositol, is reported. Benzyl protected myo-inositol was first prepared and then transformed to phosphodiester derivatives using a phosphoramidite based-coupling reaction with archaeol. Aqueous dispersions of DoPhPI alone or mixed with DoPhPC can be extruded and form small unilamellar vesicles, as detected by DLS. Neutron, SAXS, and solid-state NMR demonstrated that the water dispersions could form a lamellar phase at room temperature that then evolves into cubic and hexagonal phases with increasing temperature. Phytanyl chains were also found to impart remarkable and nearly constant dynamics to the bilayer over wide temperature ranges. All these new properties of archaeal lipids are proposed as providers of plasticity and thus means for the archaeal membrane to resist extreme conditions.Read less <
English Keywords
MAGNETIC-RESONANCE
SPECTROMETER IN15
PHASE
BILAYERS
PECTROSCOPY
TEMPERATURE
PRESSURE
SYSTEMS
ORDER
NMR