VALENCIA-GALLARDO, Cesar; AGUILAR-SALVADOR, Daniel-Isui; KHAKZAD, Hamed; COCOM-CHAN, Benjamin; BOU-NADER, Charles; VELOURS, Christophe; ZARROUK, Yosra; LE CLAINCHE, Christophe; MALOSSE, Christian; LIMA, Diogo Borges; QUENECH’DU, Nicole; MAZHAR, Bilal; ESSID, Sami; FONTECAVE, Marc; ASNACIOS, Atef; CHAMOT-ROOKE, Julia; MALMSTRÖM, Lars; TRAN VAN NHIEU, Guy

doi:10.1016/j.celrep.2023.112405

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VALENCIA-GALLARDO, Cesar
Centre interdisciplinaire de recherche en biologie [CIRB]
Labex MemoLife

AGUILAR-SALVADOR, Daniel-Isui
Centre interdisciplinaire de recherche en biologie [CIRB]
Laboratoire de biologie et pharmacologie appliquée [LBPA]

KHAKZAD, Hamed
Centre interdisciplinaire de recherche en biologie [CIRB]
Laboratoire de biologie et pharmacologie appliquée [LBPA]

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Article de revue

Ce document a été publié dans

Cell Reports. 2023-04, vol. 42, n° 4, p. 112405

Résumé en anglais

Upon activation, vinculin reinforces cytoskeletal anchorage during cell adhesion. Activating ligands classically disrupt intramolecular interactions between the vinculin head and tail domains that bind to actin filaments. Here, we show that Shigella IpaA triggers major allosteric changes in the head domain, leading to vinculin homo-oligomerization. Through the cooperative binding of its three vinculin-binding sites (VBSs), IpaA induces a striking reorientation of the D1 and D2 head subdomains associated with vinculin oligomerization. IpaA thus acts as a catalyst producing vinculin clusters that bundle actin at a distance from the activation site and trigger the formation of highly stable adhesions resisting the action of actin relaxing drugs. Unlike canonical activation, vinculin homo-oligomers induced by IpaA appear to keep a persistent imprint of the activated state in addition to their bundling activity, accounting for stable cell adhesion independent of force transduction and relevant to bacterial invasion.< Réduire

Mots clés en anglais

Shigella

invasion

vinculin

adhesion

actin

bundling

URI

https://oskar-bordeaux.fr/handle/20.500.12278/199299

DOI

http://dx.doi.org/10.1016/j.celrep.2023.112405

Project ANR

Memory in living systems: an integrated approach - ANR-10-LABX-0054

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234