MOURER, Thierry; EL GHALID, Mennat; PEHAU-ARNAUDET, Gerard; KAUFFMANN, Brice; LOQUET, Antoine; BRULE, Sebastien; CABRAL, Vitor; D’ENFERT, Christophe; BACHELLIER-BASSI, Sophie

doi:10.1038/s41522-023-00371-x

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MOURER, Thierry

EL GHALID, Mennat

PEHAU-ARNAUDET, Gerard

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Article de revue

Ce document a été publié dans

npj Biofilms and Microbiomes. 2023-12, vol. 9, n° 1, p. 6

Résumé en anglais

The human commensal fungus Candida albicans can attach to epithelia or indwelling medical devices and form biofilms, that are highly tolerant to antifungal drugs and can evade the immune response. The cell surface protein Pga59 has been shown to influence adhesion and biofilm formation. Here, we present evidence that Pga59 displays amyloid properties. Using electron microscopy, staining with an amyloid fibre-specific dye and X-ray diffraction experiments, we showed that the predicted amyloid-forming region of Pga59 is sufficient to build up an amyloid fibre in vitro and that recombinant Pga59 can also adopt a cross-β amyloid fibre architecture. Further, mutations impairing Pga59 amyloid assembly led to diminished adhesion to substrates and reduced biofilm production. Immunogold labelling on amyloid structures extracted from C. albicans revealed that Pga59 is used by the fungal cell to assemble amyloids within the cell wall in response to adhesion. Altogether, our results suggest that Pga59 amyloid properties are used by the fungal cell to mediate cell-substrate interactions and biofilm formation.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/199215

DOI

http://dx.doi.org/10.1038/s41522-023-00371-x

Projet Européen

Pathogenomics and Systems Biology of Fungal Infections - An Integrative Approach

Project ANR

Integrative Biology of Emerging Infectious Diseases - ANR-10-LABX-0062
Développement d'une infrastructure française distribuée coordonnée

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248