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The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans

dc.rights.licenseopenen_US
dc.contributor.authorMOURER, Thierry
dc.contributor.authorEL GHALID, Mennat
dc.contributor.authorPEHAU-ARNAUDET, Gerard
hal.structure.identifierSoutien à la Recherche de l'Institut Européen de Chimie Biologique
dc.contributor.authorKAUFFMANN, Brice
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLOQUET, Antoine
dc.contributor.authorBRULE, Sebastien
dc.contributor.authorCABRAL, Vitor
dc.contributor.authorD’ENFERT, Christophe
dc.contributor.authorBACHELLIER-BASSI, Sophie
dc.date.accessioned2024-04-18T08:08:29Z
dc.date.available2024-04-18T08:08:29Z
dc.date.issued2023-12
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199215
dc.description.abstractEnThe human commensal fungus Candida albicans can attach to epithelia or indwelling medical devices and form biofilms, that are highly tolerant to antifungal drugs and can evade the immune response. The cell surface protein Pga59 has been shown to influence adhesion and biofilm formation. Here, we present evidence that Pga59 displays amyloid properties. Using electron microscopy, staining with an amyloid fibre-specific dye and X-ray diffraction experiments, we showed that the predicted amyloid-forming region of Pga59 is sufficient to build up an amyloid fibre in vitro and that recombinant Pga59 can also adopt a cross-β amyloid fibre architecture. Further, mutations impairing Pga59 amyloid assembly led to diminished adhesion to substrates and reduced biofilm production. Immunogold labelling on amyloid structures extracted from C. albicans revealed that Pga59 is used by the fungal cell to assemble amyloids within the cell wall in response to adhesion. Altogether, our results suggest that Pga59 amyloid properties are used by the fungal cell to mediate cell-substrate interactions and biofilm formation.
dc.description.sponsorshipIntegrative Biology of Emerging Infectious Diseases - ANR-10-LABX-0062en_US
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée coordonnéeen_US
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enThe Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41522-023-00371-xen_US
dc.subject.halSciences du Vivant [q-bio]en_US
dc.description.sponsorshipEuropePathogenomics and Systems Biology of Fungal Infections - An Integrative Approachen_US
bordeaux.journalnpj Biofilms and Microbiomesen_US
bordeaux.page6en_US
bordeaux.volume9en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierpasteur-04017273
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=npj%20Biofilms%20and%20Microbiomes&rft.date=2023-12&rft.volume=9&rft.issue=1&rft.spage=6&rft.epage=6&rft.au=MOURER,%20Thierry&EL%20GHALID,%20Mennat&PEHAU-ARNAUDET,%20Gerard&KAUFFMANN,%20Brice&LOQUET,%20Antoine&rft.genre=article


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