JAYOL, Aurelie; DUBOIS, Veronique; POIREL, Laurent; NORDMANN, Patrice

doi:10.1128/JCM.00918-16

JAYOL, Aurelie
Microbiologie Fondamentale et Pathogénicité [MFP]

DUBOIS, Veronique
Microbiologie Fondamentale et Pathogénicité [MFP]

POIREL, Laurent
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Fribourg = University of Fribourg [UNIFR]
Centre National de Référence Associé de la Résistance aux Antibiotiques [Hôpital Bicêtre AP-HP] [CNRARA/Service de Microbiologie]
Infection, Anti-microbiens, Modélisation, Evolution [IAME (UMR_S_1137 / U1137)]

Langue

Article de revue

Ce document a été publié dans

Journal of Clinical Microbiology. 2016-09-01, vol. 54, n° 9, p. 2273-7

Résumé en anglais

Enterobacterial strains resistant to polymyxins are being increasingly reported worldwide. The conventional methods for detection of colistin-resistant isolates such as broth microdilution remain time-consuming (24 to 48 h), and methods such as disc diffusion and Etest are not reliable. Recently, the rapid polymyxin NP test was developed for rapid identification of polymyxin-resistant Enterobacteriaceae This test is based on the detection of glucose metabolism related to bacterial growth in the presence of a defined concentration of colistin (or polymyxin B). The formation of acid metabolites is evidenced by a color change of a pH indicator (red phenol) in less than 2 h. In this study, the polymyxin NP test was evaluated for detection of colistin-resistant Enterobacteriaceae directly from blood cultures. The test was performed with 73 blood culture sets (either spiked or clinical blood cultures) with various enterobacterial species. The test exhibited excellent discrimination between polymyxin-resistant and polymyxin-susceptible enterobacterial isolates, and results are obtained from blood cultures within 4 h. It is easy to perform and requires neither subculture nor a centrifugation step. This test is rapid, specific, and sensitive and allows early identification of polymyxin-resistant Enterobacteriaceae directly from blood cultures.< Réduire

Mots clés en anglais

Anti-Bacterial Agents

Blood Culture

Color

Drug Resistance

Bacterial

Enterobacteriaceae

Enterobacteriaceae Infections

Humans

Hydrogen-Ion Concentration

Indicators and Reagents

Microbial Sensitivity Tests

Polymyxins

Sensitivity and Specificity

Time Factors

URI

https://oskar-bordeaux.fr/handle/20.500.12278/186340

DOI

http://dx.doi.org/10.1128/JCM.00918-16

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

Voir/Ouvrir

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Rapid Detection of Polymyxin-Resistant Enterobacteriaceae from Blood Cultures.

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche