Viral highway to nucleus exposed by image correlation analyses.
Langue
EN
Article de revue
Ce document a été publié dans
Scientific Reports. 2018-01-18, vol. 8, n° 1, p. 1152
Résumé en anglais
Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation ...Lire la suite >
Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation methods including auto-, pair- and cross-correlation, and number and brightness analysis, to study the parvovirus entry pathway at the single-particle level in living cells. Our results show that the endosome-associated movement of virus particles fluctuates from fast to slow. Fast transit of single cytoplasmic capsids to the nuclear envelope is followed by slow movement of capsids and fast diffusion of capsid fragments in the nucleoplasm. The unique combination of image analyses allowed us to follow the fate of intracellular single virus particles and their interactions with importin β revealing previously unknown dynamics of the entry pathway.< Réduire
Mots clés en anglais
Active Transport
Cell Nucleus
Animals
Capsid
Capsid Proteins
Cats
Cell Line
Cell Nucleus
Cytosol
Epithelial Cells
Fluorescent Dyes
Gene Expression
Green Fluorescent Proteins
Image Processing
Computer-Assisted
Microscopy
Atomic Force
Microscopy
Confocal
Oocytes
Organic Chemicals
Parvovirus
Canine
Spectrometry
Fluorescence
Virion
Xenopus laevis
beta Karyopherins
Unités de recherche