Viral highway to nucleus exposed by image correlation analyses.
| dc.rights.license | open | en_US |
| dc.contributor.author | MÄNTYLÄ, Elina | |
| dc.contributor.author | CHACKO, Jenu V | |
| dc.contributor.author | AHO, Vesa | |
| dc.contributor.author | PARRISH, Colin R | |
| dc.contributor.author | SHAHIN, Victor | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | KANN, Michael | |
| dc.contributor.author | DIGMAN, Michelle A | |
| dc.contributor.author | GRATTON, Enrico | |
| dc.contributor.author | VIHINEN-RANTA, Maija | |
| dc.date.accessioned | 2023-11-20T15:49:11Z | |
| dc.date.available | 2023-11-20T15:49:11Z | |
| dc.date.issued | 2018-01-18 | |
| dc.identifier.issn | 2045-2322 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184916 | |
| dc.description.abstractEn | Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation methods including auto-, pair- and cross-correlation, and number and brightness analysis, to study the parvovirus entry pathway at the single-particle level in living cells. Our results show that the endosome-associated movement of virus particles fluctuates from fast to slow. Fast transit of single cytoplasmic capsids to the nuclear envelope is followed by slow movement of capsids and fast diffusion of capsid fragments in the nucleoplasm. The unique combination of image analyses allowed us to follow the fate of intracellular single virus particles and their interactions with importin β revealing previously unknown dynamics of the entry pathway. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Active Transport | |
| dc.subject.en | Cell Nucleus | |
| dc.subject.en | Animals | |
| dc.subject.en | Capsid | |
| dc.subject.en | Capsid Proteins | |
| dc.subject.en | Cats | |
| dc.subject.en | Cell Line | |
| dc.subject.en | Cell Nucleus | |
| dc.subject.en | Cytosol | |
| dc.subject.en | Epithelial Cells | |
| dc.subject.en | Fluorescent Dyes | |
| dc.subject.en | Gene Expression | |
| dc.subject.en | Green Fluorescent Proteins | |
| dc.subject.en | Image Processing | |
| dc.subject.en | Computer-Assisted | |
| dc.subject.en | Microscopy | |
| dc.subject.en | Atomic Force | |
| dc.subject.en | Microscopy | |
| dc.subject.en | Confocal | |
| dc.subject.en | Oocytes | |
| dc.subject.en | Organic Chemicals | |
| dc.subject.en | Parvovirus | |
| dc.subject.en | Canine | |
| dc.subject.en | Spectrometry | |
| dc.subject.en | Fluorescence | |
| dc.subject.en | Virion | |
| dc.subject.en | Xenopus laevis | |
| dc.subject.en | beta Karyopherins | |
| dc.title.en | Viral highway to nucleus exposed by image correlation analyses. | |
| dc.title.alternative | Sci Rep | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1038/s41598-018-19582-w | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 29348472 | en_US |
| bordeaux.journal | Scientific Reports | en_US |
| bordeaux.page | 1152 | en_US |
| bordeaux.volume | 8 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 1 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04296317 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-11-20T15:49:14Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2018-01-18&rft.volume=8&rft.issue=1&rft.spage=1152&rft.epage=1152&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=M%C3%84NTYL%C3%84,%20Elina&CHACKO,%20Jenu%20V&AHO,%20Vesa&PARRISH,%20Colin%20R&SHAHIN,%20Victor&rft.genre=article |
