MARIE, Lea; RAPISARDA, Chiara; MORALES, Violette; BERGE, Mathieu; PERRY, Thomas; SOULET, Anne-Lise; GRUGET, Clemence; REMAUT, Han; FRONZES, Remi; POLARD, Patrice

doi:10.1038/ncomms15638

RAPISARDA, Chiara
Microbiologie Fondamentale et Pathogénicité [MFP]

MORALES, Violette
Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM]

Langue

Article de revue

Ce document a été publié dans

Nature Communications. 2017-05-31, vol. 8, p. 15638

Résumé en anglais

Homologous recombination (HR) is a central process of genome biology driven by a conserved recombinase, which catalyses the pairing of single-stranded DNA (ssDNA) with double-stranded DNA to generate a D-loop intermediate. Bacterial RadA is a conserved HR effector acting with RecA recombinase to promote ssDNA integration. The mechanism of this RadA-mediated assistance to RecA is unknown. Here, we report functional and structural analyses of RadA from the human pathogen Streptococcus pneumoniae. RadA is found to facilitate RecA-driven ssDNA recombination over long genomic distances during natural transformation. RadA is revealed as a hexameric DnaB-type helicase, which interacts with RecA to promote orientated unwinding of branched DNA molecules mimicking D-loop boundaries. These findings support a model of DNA branch migration in HR, relying on RecA-mediated loading of RadA hexamers on each strand of the recipient dsDNA in the D-loop, from which they migrate divergently to facilitate incorporation of invading ssDNA.< Réduire

Mots clés en anglais

Bacterial Proteins

Crystallography

X-Ray

DNA Helicases

DNA

Single-Stranded

DNA-Binding Proteins

DnaB Helicases

Homologous Recombination

Mutagenesis

Site-Directed

Protein Domains

Protein Structure

Quaternary

Rec A Recombinases

Recombination

Genetic

Streptococcus pneumoniae

Two-Hybrid System Techniques

URI

https://oskar-bordeaux.fr/handle/20.500.12278/184853

DOI

http://dx.doi.org/10.1038/ncomms15638

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

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Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension.

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Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche